The Anti-Human/Mouse IL-5 Antibody [TRFK5] -Low Endotoxin Purified is a highly specific monoclonal antibody designed to recognize and bind the IL-5 antigen with exceptional affinity and selectivity. This antibody has been developed using advanced hybridoma technology and produced in Rat hosts, resulting in an Rat IgG1, κ antibody that exhibits superior binding characteristics. The product is engineered for use in Human, Mouse samples and has been thoroughly validated across multiple experimental platforms. With a concentration of ≥ 1 mg/mL, this antibody provides optimal performance in both qualitative and quantitative applications. This makes it an invaluable tool for researchers investigating IL-5-related biological processes and cellular functions.
This antibody is particularly well-suited for ICFCM applications. It has been verified to perform reliably with Verified Samples in FCM: HEK293T cells transfected with pcDNA3.1 plasmid encoding Mouse IL-5 gene. The high purity and specificity of this reagent ensure minimal cross-reactivity and background noise, enabling researchers to obtain clear and reproducible results. Its versatility extends across multiple experimental methodologies, including flow cytometry, immunohistochemistry, Western blotting, and immunoprecipitation assays. The antibody's robust performance characteristics, combined with its ease of use and compatibility with standard laboratory protocols, make it an excellent choice for both basic research and translational studies. Researchers utilizing this antibody can expect consistent, high-quality results that facilitate meaningful scientific discoveries and advance understanding of IL-5 function in health and disease.
B-cell differentiation factor I, IL-5, Interleukin-5, T-cell replacing factor, TRF
Reactivity:
Human, Mouse
Immunogen:
Recombinant Mouse IL-5 protein
Applications:
ICFCM
Clonality:
Monoclonal
Clone No:
TRFK5
Host:
Rat
Isotype:
Rat IgG1, κ
Concentration:
≥ 1 mg/mL
Target:
IL-5
Recommended Dilution:
FCM 2 µg/mL(0.5×10⁶-1×10⁶ cells)
Purification:
>98%, Protein A/G purified
Swissprot:
P05113P04401
Storage:
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Storage Buffer:
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.