The IL13 receptor alpha 1 Monoclonal Antibody (CAB3524) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been thoroughly validated for use in Western blot applications. By binding to the IL13RA1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an invaluable resource for studies in immunology and cancer research.IL13RA1, also known as interleukin-13 receptor alpha 1, is a crucial component in the signaling pathway of interleukin-13, a cytokine involved in inflammation and immune regulation.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
IL13 receptor alpha 1 Monoclonal Antibody
SKU:
CAB3524
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2027
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NR4, CT19, CD213A1, IL-13Ra, IL13RA1
Positive Sample:
HeLa, Hep G2, A549
Cellular Localization:
External Side Of Plasma Membrane, Plasma Membrane.
Calculated MW:
49kDa
Observed MW:
49kDa
The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.
Purification Method
Affinity purification
Gene ID
3597
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using IL13RA1 Rabbit mAb (CAB3524) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using IL13RA1 Rabbit mAb (CAB3524) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
