The INTS8 Antibody (CAB20513) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and is validated for use in Western blot and immunofluorescence applications. By binding to INTS8, the antibody allows for the visualization and analysis of this protein in various cell types, making it particularly useful for studies in molecular biology and gene expression regulation.INTS8 is essential for the proper functioning of the Integrator complex, which plays a crucial role in processing non-coding RNAs and regulating gene expression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
INTS8 Antibody
SKU:
CAB20513
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
INT8, NEDCHS, C8orf52, INTS8
Positive Sample:
Mouse brain
Cellular Localization:
Nucleoplasm, Nucleus.
Calculated MW:
113kDa
Observed MW:
90-115kDa
This gene encodes a subunit of the Integrator complex which is involved in the cleavage of small nuclear RNAs U1 and U2 within the nucleus. The encoded protein associates with RNA polymerase II and is recruited to the U1 and U2 small nuclear RNA genes. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
55656
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse brain, using INTS8 Rabbit pAb (CAB20513) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
