The [KO Validated] ITCH Monoclonal Antibody (CAB8624) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, validated for Knockout (KO) studies, is highly specific and sensitive for detecting anti-itch molecules in various cell types. It is raised in rabbits and is proven to be effective in Western blot applications for analyzing the expression levels of anti-itch proteins.The anti-itch antibody targets key molecules involved in itch sensation and signaling pathways, providing a valuable tool for studying the mechanisms underlying itch perception and potential therapeutic targets for treating itch-related disorders such as eczema, psoriasis, and allergic reactions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] ITCH Monoclonal Antibody
SKU:
CAB8624
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1759
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
83737
RRID
AB_2863575
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ITCH Rabbit mAb (CAB8624) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using ITCH Rabbit mAb (CAB8624) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
