Lactate Dehydrogenase C Monoclonal Antibody (CAB20866)
The Lactate Dehydrogenase C Monoclonal Antibody (CAB20866) is a high-quality antibody developed for reliable detection and analysis of target proteins. LDH-C is crucial for the conversion of lactate to pyruvate during anaerobic respiration, making it a key player in metabolic processes.This polyclonal antibody, raised in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By binding specifically to the LDH-C protein, researchers can accurately detect and analyze this enzyme in a variety of cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Lactate Dehydrogenase C Monoclonal Antibody
SKU:
CAB20866
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2796
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CT32, LDH3, LDHX, Lactate Dehydrogenase C
Positive Sample:
Human testis
Cellular Localization:
Cytoplasm.
Calculated MW:
36kDa
Observed MW:
36kDa
Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region.
Purification Method
Affinity purification
Gene ID
3948
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Lactate Dehydrogenase C Rabbit mAb (CAB20866) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
