The LHX2 Monoclonal Antibody (CAB19265) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, boasts high reactivity with human samples and has been validated for use in various applications, including Western blotting.LHX2 plays a crucial role in regulating gene expression and controlling cell fate decisions, making it a key player in developmental biology and stem cell research. The antibody specifically binds to the LHX2 protein, enabling researchers to detect and analyze its presence in different cell types with precision and accuracy.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LHX2 Monoclonal Antibody
SKU:
CAB19265
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2435
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LH2, hLhx2, LHX2
Positive Sample:
Neuro-2a, Rat brain, NCI-H524, A-431, Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
44kDa
Observed MW:
44kDa
This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution.
Purification Method
Affinity purification
Gene ID
9355
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using LHX2 Rabbit mAb (CAB19265) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Western blot analysis of various lysates using LHX2 Rabbit mAb (CAB19265) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
