The LysRS/KARS Monoclonal Antibody (CAB8648) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been shown to be highly specific for human samples and is validated for use in Western blot applications. By binding to the LYSRS/KARS protein, this antibody allows for the detection and analysis of this crucial molecule in various cell types, making it an ideal choice for studies in immunology and cancer research.The LYSRS/KARS protein, also known as lysyl-tRNA synthetase, plays a key role in protein synthesis and immune regulation.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LysRS/KARS Monoclonal Antibody
SKU:
CAB8648
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1765
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
3735
RRID
AB_2863576
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using LysRS/KARS Rabbit mAb (CAB8648) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using LysRS/KARS Rabbit mAb (CAB8648) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of C6 cells using LysRS/KARS Rabbit mAb (CAB8648) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using LysRS/KARS Rabbit mAb (CAB8648) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation of LysRS/KARS from 300µg µg extracts of 293F cells was performed using 3µg µg of LysRS/KARS Rabbit mAb (CAB8648). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using () at a dilution of 1:3000.
