The MBD2 Monoclonal Antibody (CAB4032) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is validated for Western blot applications. By binding to the MBD2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in epigenetics, cancer research, and developmental biology.MBD2, also known as methyl-CpG binding domain protein 2, is involved in the recognition and interpretation of DNA methylation patterns, playing a crucial role in gene expression regulation and chromatin structure modulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
MBD2 Monoclonal Antibody
SKU:
CAB4032
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC2691
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DMTase, NY-CO-41, MBD2
Positive Sample:
Mouse liver
Cellular Localization:
Cytosol, Nucleoplasm, Nucleus.
Calculated MW:
43kDa
Observed MW:
46kDa
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
8932
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse liver , using MBD2 Rabbit mAb (CAB4032) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180 s.
