The MELK Monoclonal Antibody (CAB3530) is a high-quality antibody developed for reliable detection and analysis of target proteins. MELK is a key player in cell cycle progression and is known to be upregulated in various types of cancer, making it a potential target for cancer therapy.This polyclonal antibody, generated in rabbits, is validated for use in immunohistochemistry and Western blot applications, as well as immunoprecipitation assays. It binds specifically to the MELK protein, enabling accurate detection and quantification in cell lysates and tissue samples.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MELK Monoclonal Antibody
SKU:
CAB3530
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2032
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HPK38, MELK
Positive Sample:
293T, Jurkat, K-562, Mouse testis, Rat testis
Cellular Localization:
Cell Membrane, Peripheral Membrane Protein.
Calculated MW:
75kDa
Observed MW:
75kDa
Enables calcium ion binding activity; non-membrane spanning protein tyrosine kinase activity; and protein serine/threonine kinase activity. Involved in apoptotic process; cell population proliferation; and protein autophosphorylation. Located in cell cortex and plasma membrane.
Purification Method
Affinity purification
Gene ID
9833
RRID
AB_2863080
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MELK Rabbit mAb (CAB3530) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
