The MonoMethyl-Histone H3-K18 Monoclonal Antibody (CAB20680) is a high-quality antibody developed for reliable detection and analysis of target proteins. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ChIP, ELISA, DB, CUT&Tag applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
MonoMethyl-Histone H3-K18 Monoclonal Antibody
SKU:
CAB20680
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Clone Number:
ARC2621
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCIPChIPELISADBCUT&Tag
Recommended Dilution:
WB
1:500 - 1:1000
DB
1:500 - 1:1000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
IP
2μg-6μg antibody for 400μg-600μg extracts of whole cells
ChIP
5μg antibody for 5μg-10μg of Chromatin
CUT&Tag
10⁵ cells /1 μg
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa nuclear extract, NIH/3T3 nuclear extract, C6 nuclear extract
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
15 kDa
Observed MW:
17 kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H3-K18 Rabbit mAb (CAB20680) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Dot-blot analysis of all sorts of peptides using MonoMethyl-Histone H3-K18 antibody (CAB20680) at 1:1000 dilution.
Immunohistochemistry analysis of paraffin-embedded Mouse lung using MonoMethyl-Histone H3-K18 Rabbit mAb (CAB20680) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain using MonoMethyl-Histone H3-K18 Rabbit mAb (CAB20680) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H3-K18 Rabbit mAb (CAB20680) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600 μg extracts of 293F cells using 5 μg MonoMethyl-Histone H3-K18 antibody (CAB20680). Western blot was performed from the immunoprecipitate using MonoMethyl-Histone H3-K18 antibody (CAB20680) at a dilution of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K18 antibody (CAB20680) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
