Description
Anti-Mouse CD11c In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD11c In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody optimized for in vivo applications. This antibody targets the CD11c protein, an essential integrin involved in the immune response, making it ideal for research in immunology and related fields. With a rat IgG1 isotype, it guarantees high purity and low endotoxin levels (<1.0 EU/mg), making it perfect for flow cytometry, immunohistochemistry, immunofluorescence, and in vivo functional assays.
Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Elevate your research with this reliable and versatile antibody. CD11c is a transmembrane protein predominantly expressed on dendritic cells, macrophages, and some subsets of T-cells and B-cells. It plays a crucial role in the immune system by mediating cell adhesion and signaling necessary for the activation and migration of immune cells, contributing to the body's defense mechanisms.
| Product Name: | Anti-Mouse CD11c In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0141 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | N418 |
| Protein: | CD11c |
| Product Type: | Monoclonal Antibody |
| Synonyms: | αX integrin, integrin αX chain, CR4, p150, ITGAX |
| Isotype: | Armenian Hamster IgG |
| Reactivity: | Mouse |
| Immunogen: | Mouse spleen dendritic cells |
| Applications: | B, CyTOF®, IHC FF, PhenoCycler®, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CODEX®, CyTOF®, IHC FF, WB |
| Recommended Usage: | FC The suggested concentration for this N418 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this N418 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Armenian Hamster |
| Specificity: | Clone N418 recognizes an epitope on mouse CD11C. |
| Antigen Distribution: | CD11c is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. |
| Immunogen: | Mouse spleen dendritic cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
| Technical Datasheet: | View |
| Protein: | CD11c |
| Function: | Cellular adhesion |
| Ligand/Receptor: | iC3b, fibrinogen |
| Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers |
| Armenian Hamster IgG Isotype Control | |
|---|---|
| Clone | PIP |
| Isotype | Armenian Hamster IgG |
| Endotoxin Level | Low Endotoxin |
