Description
Anti-Mouse CD127 (IL-7Rα) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD127 (IL-7R) In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the IL-7 receptor (CD127), a critical component in the development and maintenance of the immune system, making it ideal for research in immunology and related fields.
With its mouse IgG2a isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Enhance your research with this reliable and versatile antibody. CD127, also known as the interleukin-7 receptor alpha chain (IL-7Rα), is expressed on the surface of various immune cells, including T cells, B cells, and monocytes. It plays a significant role in lymphocyte development and homeostasis by influencing cell survival, proliferation, and differentiation through interaction with interleukin-7 (IL-7).
| Product Name: | Anti-Mouse CD127 (IL-7Rα) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0044 |
| Size: | 1mg |
| Clone: | A7R34 |
| Protein: | CD127 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | IL-7Rα, Interleukin-7 receptor alpha, IL-7Rα chain |
| Isotype: | Rat IgG2a κ |
| Reactivity: | Mouse |
| Immunogen: | IL-7Ra-IgG1 fusion protein |
| Applications: | B, CyTOF®, FA, FC, IF Staining, IHC FF, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CyTOF®, FA, FC, IF Staining, IHC FF, IP, WB |
| Recommended Usage: | FC The suggested concentration for this A7R34 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone A7R34 recognizes an epitope on mouse CD127. |
| Antigen Distribution: | IL-7Rα is expressed on bone marrow lymphoid precursors, pro-B cells, monocytes/macrophages, NK-cells, naïve T cells, memory CD4+ and CD8+ T cells, intestinal colorectal and renal carcinoma cell lines, and keratinocytes. |
| Immunogen: | IL-7Ra-IgG1 fusion protein |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD127 (IL7R-α) is a 60-90 kD type I cytokine receptor involved in the regulation of lymphopoiesis. The active receptor is an α/γ chain heterodimer. The common γ chain, which also associates with the IL-2 receptor, serves mainly to activate signal transduction via the IL7R complex. CD127 also interacts with the Thymic Stromal Lymphopoietin Receptor (TSLPR). CD127 determines signaling events via its association with cytoplasmic signaling molecules and has been reported to be a useful marker for identifying memory and effector T cells.
| Protein: | CD127 |
| Function: | T cell and immature B cell proliferation and development |
| Ligand/Receptor: | IL-7 |
| Research Area: | Immunology |
