Description
Anti-Mouse CD278 (ICOS) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD278 (ICOS) In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody tailored for in vivo applications. This antibody targets the CD278 (Inducible T-cell COStimulator or ICOS) protein, pivotal in immune regulation and T-cell activation, making it indispensable for research in immunology and related fields.
With a rat IgG2a isotype, it guarantees high purity and low endotoxin levels (<1.0 EU/mg), ensuring suitability for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in multiple sizes, it is formulated in phosphate-buffered saline for enhanced stability and efficacy. Elevate your research with this dependable and versatile antibody. CD278 (ICOS) is a co-stimulatory molecule expressed on the surface of T-cells, playing a critical role in T-cell proliferation, differentiation, and survival. It enhances immune responses by binding to its ligand, ICOSL, on antigen-presenting cells. This interaction is vital for the regulation of immune responses, making the Anti-Mouse CD278 (ICOS) antibody a powerful tool for studies in immune modulation and therapy.
| Product Name: | Anti-Mouse CD278 (ICOS) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0082 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 7E.17G9 |
| Protein: | CD278 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Inducible Costimulator, ICOS |
| Isotype: | Rat IgG2b κ |
| Reactivity: | Mouse |
| Immunogen: | Mouse ICOS cDNA and ICOS hexahistidine fusion protein |
| Applications: | B, FA, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, FA, WB |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone 7E.17G9 recognizes an epitope on mouse CD278. |
| Antigen Distribution: | ICOS is expressed on activated T cells and APCs including splenic B cells, dendritic cells, and macrophages. |
| Immunogen: | Mouse ICOS cDNA and ICOS hexahistidine fusion protein |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD278 (ICOS) belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers (47-57kD) and enhances all basic T cell responses to a foreign antigen, specifically proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B cells. ICOS signaling is also an important regulator for T cell homeostasis. ICOS plays a role in humoral immune response and is thought to have involvement in the development of Th2 cells and to play a protective role in inflammatory autoimmune diseases.
| Technical Datasheet: | View |
| Protein: | CD278 |
| Function: | Costimulates T cell activation, proliferation, humoral immune response |
| Ligand/Receptor: | B7h/B7RP-1/GL-50 |
| Research Area: | Costimulatory Molecules, Immunology |
