Description
Anti-Mouse CD278 (ICOS) In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse CD278 (ICOS) In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD278 protein, also known as Inducible T-cell COStimulator (ICOS), which is critical in T-cell activation and survival, making it an essential tool for research in immunology and related fields. With a rat IgG1 isotype, it ensures high purity and ultra-low endotoxin levels (<0.002 EU/μg), making it ideal for applications such as ELISA, flow cytometry, functional assays, and in vivo studies. As one of the pivotal co-stimulatory molecules on the surface of T-cells, ICOS plays a vital role in the immune regulatory pathways by enhancing T-cell responses, promoting cytokine production, and supporting T-cell proliferation.
This antibody is expertly formulated in a phosphate-buffered saline solution for enhanced stability and efficacy. Available in multiple sizes to suit your specific research needs, the Anti-Mouse CD278 (ICOS) In Vivo Antibody is your reliable partner in advancing scientific understanding and innovation in immunological research. Enhance the precision and impact of your experiments with this robust and versatile in vivo antibody.
| Product Name: | Anti-Mouse CD278 (ICOS) In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0083 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 7E.17G9 |
| Protein: | CD278 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Inducible Costimulator,ICOS |
| Isotype: | Rat IgG2b |
| Reactivity: | Mouse |
| Immunogen: | Mouse ICOS cDNA and ICOS hexahistidine fusion protein |
| Applications: | B, FA, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, FA, WB |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone 7E.17G9 recognizes an epitope on mouse CD278. |
| Antigen Distribution: | ICOS is expressed on activated T cells and APCs including splenic B cells, dendritic cells, and macrophages. |
| Immunogen: | Mouse ICOS cDNA and ICOS hexahistidine fusion protein |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD278 (ICOS) belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers (47-57kD) and enhances all basic T cell responses to a foreign antigen, specifically proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B cells. ICOS signaling is also an important regulator for T cell homeostasis. ICOS plays a role in humoral immune response and is thought to have involvement in the development of Th2 cells and to play a protective role in inflammatory autoimmune diseases.
| Technical Datasheet: | View |
| Protein: | CD278 |
| Ligand/Receptor: | B7h/B7RP-1/GL-50 |
| Research Area: | Costimulatory Molecules, Immunology |
| Rat IgG2b Isotype Control | |
|---|---|
| Clone | 1-2 |
| Isotype | Rat IgG2b kappa |
| Endotoxin Level | Ultra Low Endotoxin |
