Description
Anti-Mouse CTLA-4 (CD152) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CTLA-4 (CD152) In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody meticulously designed for in vivo applications.
This antibody targets the CTLA-4 protein, a pivotal immune checkpoint receptor involved in downregulating immune responses, making it indispensable for research in immunology, particularly in the study of T-cell functions and immune regulation. With a rat IgG isotype, this antibody guarantees high purity and low endotoxin levels (<1.0 EU/mg), ensuring optimal performance in various applications including ELISA, flow cytometry, and functional assays. Available in multiple sizes, it is formulated in phosphate-buffered saline to maintain stability and efficacy. Elevate your research potential with this dependable and versatile antibody. CTLA-4, also known as CD152, is expressed on the surface of activated T-cells and regulatory T-cells (Tregs). It is crucial in maintaining immune homeostasis by delivering inhibitory signals to T-cells, thereby preventing overactivation and autoimmunity. By targeting this key regulatory molecule, the Anti-Mouse CTLA-4 In Vivo Antibody from Assay Genie provides a powerful tool for advancing your research in immune modulation and therapeutic interventions.
| Product Name: | Anti-Mouse CTLA-4 (CD152) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0049 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | UC10-4F10-11 |
| Protein: | CTLA-4 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | CD152, Cytotoxic T Lymphocyte-Associated Antigen-4, Ly-56 |
| Isotype: | Armenian Hamster IgG1 κ |
| Reactivity: | Mouse |
| Immunogen: | Purified Recombinant Mouse CTLA4 Fc Fusion Protein |
| Applications: | FA, FC, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FA, FC, IP, WB |
| Reactivity: | Mouse |
| Host Species: | Armenian Hamster |
| Specificity: | Clone UC10-4F10-11 recognizes an epitope on mouse CTLA-4. |
| Antigen Distribution: | CTLA-4 is expressed on activated T and B lymphocytes. |
| Immunogen: | Purified Recombinant Mouse CTLA4 Fc Fusion Protein |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CTLA-4 is a 33 kD member of the Ig superfamily similar to CD28 in amino acid sequence, structure, and genomic organization. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses. It is involved in the development of protective immunity and thymocyte regulation, in addition to the induction and maintenance of immunological tolerance. CTLA-4 has therapeutic potential both as an agonist to reduce immune activity, and an antagonist to increase immune activity.
| Technical Datasheet: | View |
| Protein: | CTLA-4 |
| Function: | Negative regulator of T cell activation |
| Ligand/Receptor: | CD80 (B7-1), CD86 (B7-2) |
| Research Area: | Immunology, Inhibitory Molecules |
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