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Anti-Mouse/Human KLRG-1 Monoclonal Antibody (IVMB0401)

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SKU IVMB0401
Antibody Type Biosimilar Reference Antibody
Applications IHC
Applications FC
Applications Drug Development
Applications Pharmacokinetics
Applications ICC
Applications IP
Applications WB
Disease Area Cancer
Clone 2F1
Isotype Syrian Hamster IgG
Host Species Syrian Hamster
Reactivity Human/Mouse
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Description

system_update_altDatasheet
Product Name: Anti-Mouse/Human KLRG-1 – Purified
Product Code: IVMB0401
Size: 100 μg, 500 μg
Clone: 2F1
Target: KLRG-1
Host Species: Syrian Hamster
Product Type: Hybridoma Monoclonal Antibody
Synonyms: KLRG1, 2F1, MAFA
Isotype: Syrian Hamster IgG
Reactivity: Human/Mouse
Applications: FC, ICC, IP, WB
Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Concentration: 0.5 mg/ml
State of Matter: Liquid

Killer cell lectin-like receptor G1 (KLRG1) is a transmembrane glycoprotein with an extracellular C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM)1. KLRG1 plays an inhibitory role in human NK and T cells2 and is frequently used as a marker of cellular differentiation, with memory cells and highly differentiated end-stage cells having a significantly higher percentage of expression than differentiating cells2. Additionally, the proportion of CD8+ T cells expressing KLRG1 is age-related, increasing from ~ 40% of the CD8+ T cell pool in young adults to 90% in individuals over 65. KLRG1 is known to bind E-, N-, and R-cadherin1, 2. Cadherin interactions are generally involved in cell-to-cell adhesion and additionally, KLRG1/E-cadherin interactions are involved in immune control2.

2F1 was generated by immunizing Syrian hamsters with IL-2 activated NK (A-LAK) cells from B6 (H-2b) mice3. Popliteal lymph node cells were fused to murine myeloma P3X63Ag.8.653 cells and hybridomas screened for antibodies specific to A-LAK cells.

Antibody 2F1 was used to show that KLRG1 is indirectly upregulated by expression of MHC class I molecules in an interaction affected by class I-specific Ly49 inhibitory receptors and was also used to show that KLRG1 expression is modulated by SHP-1 3 . Additionally, 2F1 was used to identify the KLRG1 mouse homolog and show that it is not expressed on mouse mast cells 4.

Specificity: 2F1 activity is directed against mouse and human KLRG1.
Antigen Distribution: KLRG1 is expressed by subsets of natural killer cells and a small fraction of T cells but not by other lymphohematopoietic cells in both mouse and human.
Immunogen: IL-2-activated NK cells (A-LAK cells) from B6 (H-2b) mice
Ligand/Receptor: E-, N-, R- cadherins
Storage and Handling: This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Uniprot: Q96E93
NCBI Gene Bank ID: 10219

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