Description
Anti-Mouse NK1.1 In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse NK1.1 In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody optimized for in vivo applications. This antibody targets the NK1.1 protein, a critical marker for natural killer (NK) cells, making it indispensable for research in immunology and related fields.
Featuring a mouse IgG2a isotype, it assures high purity and low endotoxin levels (<1.0 EU/mg), making it ideal for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for optimal stability and efficacy. Elevate your research with this dependable and versatile antibody. NK1.1, also known as NKR-P1C, is an activation receptor found predominantly on NK cells and a subset of T-cells. It plays a significant role in the immune system by mediating the cytotoxic activity of NK cells and contributing to the innate immune response.
| Product Name: | Anti-Mouse NK1.1 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0100 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | PK136 |
| Protein: | NK1.1 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Natural Killer Cells, KR-P1C, NKR-P1B, Ly-55, CD161b, CD161c |
| Isotype: | Mouse IgG2a κ |
| Reactivity: | Mouse |
| Immunogen: | NK-1+ cells from mouse spleen and bone marrow |
| Applications: | B, CyTOF®, Depletion, FC, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CyTOF®, Depletion, FC, IP, WB |
| Recommended Usage: | FC The suggested use of this antibody is ≤ 0.25 µg per 106 in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Mouse |
| Specificity: | Clone PK136 recognizes mouse NK1.1. |
| Antigen Distribution: | NK-1.1 is encoded by the NKR-P1B/NKR-P1C gene and expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. |
| Immunogen: | NK-1+ cells from mouse spleen and bone marrow |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
NK1.1 is a type II membrane protein that is part of the C-type lectin superfamily. NK1.1 contains a transmembrane domain and a cytoplasmic domain that are characteristic of C-type lectins. The function of NK cells is to mediate cytotoxicity and to secrete cytokines after immune stimulation. NK1.1 has been correlated with lysis of tumor cells In vitro and rejection of bone marrow allografts In vivo. It is also involved in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 is commonly used as a mouse NK cell marker.
| Technical Datasheet: | View |
| Protein: | NK1.1 |
| Function: | NK cell activation, IFN-γ production, cytotoxic granule release |
| Research Area: | Immunology, Innate Immunity |
| Mouse IgG2a Isotype Control | |
|---|---|
| Clone | C1.18.4 |
| Isotype | Mouse IgG2a |
| Endotoxin Level | Low Endotoxin |
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| Turley et al. | Intratumoral delivery of the chitin-derived C100 adjuvant promotes robust STING, IFNAR, and CD8+ T cell-dependent anti-tumor immunity | Cell Reports Medicine 2024 | PubMed ID: 38729159 |
| Nakawesi, J. et al. | Natural Killer Cells Are Dispensable for Virus Control in Rag2−/− Mice During Primary RSV Infection | Immunology 2025 | PubMed ID: 40899087 |
