Description
system_update_altTechnical Datasheet
| Product Name: | Anti-Mouse TCR V gamma 2 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0074 |
| Size: | 1mg, 5mg, 25mg, 100mg |
| Clone: | UC3-10A6 |
| Protein: | V-gamma 2 T-Cell Receptor |
| Product Type: | Monoclonal Antibody |
| Synonyms: | V-gamma2 T-cell receptor, T cell receptor gamma 2 |
| Isotype: | Armenian Hamster IgG |
| Reactivity: | Mouse |
| Applications: | Depletion, FC, IP |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | Depletion, FC, IP |
| Recommended Usage: | FC It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 µg per 1.0 X 106 in a 100 µl total staining volume, followed by Goat-anti-Hamster IgG. Each lot of this antibody is quality control tested. |
| Reactivity: | Mouse |
| Host Species: | Armenian Hamster |
| Specificity: | This antibody reacts with V-gamma 2 T-Cell Receptor (TCR)-bearing T lymphocytes. |
| Antigen Distribution: | V-gamma 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of γδ TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung. |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.
| Technical Datasheet: | View |
| Protein: | V-gamma 2 T-Cell Receptor |
| Function: | Studies suggest that these cells recognize bacterial ligands and some tumor cells. |
| Ligand/Receptor: | Antigens |
| Research Area: | Adaptive Immunity, Immunology |
| Armenian Hamster IgG Isotype Control | |
|---|---|
| Clone | PIP |
| Isotype | Armenian Hamster IgG |
| Endotoxin Level | Low Endotoxin |
