Description
Anti-Mouse TNFα In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse TNF In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody meticulously developed for in vivo applications. This antibody targets the tumor necrosis factor (TNF), a pivotal cytokine in inflammation and immune response, making it indispensable for research in immunology and related fields. With a mouse IgG1 isotype, it guarantees exceptional purity and ultra-low endotoxin levels (<0.1 EU/mg), ideal for ELISA, in vivo functional assays, and other sensitive experiments. Available in various sizes, it is formulated in phosphate-buffered saline for optimal stability and efficacy.
Elevate your research with this superior and versatile antibody. TNF is a critical cytokine involved in systemic inflammation and is part of the body's acute phase reaction. It is primarily produced by macrophages, T-cells, and natural killer cells and plays a crucial role in the regulation of immune cells. By targeting TNF, this antibody facilitates the detailed study of inflammatory responses and immune system dynamics, enhancing the understanding of disease mechanisms and therapeutic interventions.
| Product Name: | Anti-Mouse TNFα In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0008 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | TN3-19.12 |
| Protein: | TNF-α |
| Product Type: | Monoclonal Antibody |
| Synonyms: | TNF-alpha, TNFSF2, Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin |
| Isotype: | Armenian Hamster IgG |
| Reactivity: | Mouse |
| Immunogen: | Purified Recombinant Mouse TNF-α |
| Applications: | IP, N, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | IP, N, WB |
| Recommended Usage: | WB |
| Reactivity: | Mouse |
| Host Species: | Armenian Hamster |
| Specificity: | Clone TN3-19.12 recognizes mouse TNFα. |
| Antigen Distribution: | TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells. |
| Immunogen: | Purified Recombinant Mouse TNF-α |
| Concentration: | ≥ 2.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
| Technical Datasheet: | View |
| Protein: | TNF-α |
| Ligand/Receptor: | TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75) |
| Armenian Hamster IgG Isotype Control | |
|---|---|
| Clone | PIP |
| Isotype | Armenian Hamster IgG1 |
| Endotoxin Level | Ultra Low Endotoxin |
