The NEK7 Monoclonal Antibody (CAB19816) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in mice, is highly specific for NEK7 in human samples and is optimized for use in various experimental applications, including Western blot and immunofluorescence.NEK7 is a member of the NIMA-related kinase family and plays a crucial role in centrosome duplication, microtubule organization, and mitotic progression. Dysregulation of NEK7 has been linked to various diseases, including cancer, making it a promising target for therapeutic intervention.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NEK7 Monoclonal Antibody
SKU:
CAB19816
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2342
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NIMA-related kinase 7, NEK7
Positive Sample:
HeLa, MCF7, NIH/3T3, C6, Mouse lung, Mouse liver
Cellular Localization:
Cytoplasm, Microtubule Organizing Center, Nucleoplasm, Nucleus, Spindle Pole, Cytoskeleton, Centrosome.
Calculated MW:
35kDa
Observed MW:
32kDa
NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.
Purification Method
Affinity purification
Gene ID
140609
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NEK7 Rabbit mAb (CAB19816) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
