The NG2/CSPG4 Monoclonal Antibody (CAB21095) is a high-quality antibody developed for reliable detection and analysis of target proteins. A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. RRID Gene ID 1464 Swiss Prot Synonym NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG; NG2/CSPG4
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NG2/CSPG4 Monoclonal Antibody
SKU:
CAB21095
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC52162
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Apical Cell Membrane, Cell Projection, Extracellular Side, Single-Pass Type I Membrane Protein, Lamellipodium Membrane.
Calculated MW:
251kDa
Observed MW:
450kDa
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. RRID Gene ID 1464 Swiss Prot Synonym NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG; NG2/CSPG4
Purification Method:
Affinity purification
Gene ID:
1464
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A375 cells, using NG2/CSPG4 Rabbit mAb (CAB21095) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
