The pan-Nitrific-Y Antibody (CAB20441) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody is raised in rabbits and exhibits high reactivity with human samples, making it ideal for various experimental applications such as Western blotting.Nitrific-γ is essential for the conversion of nitrite to nitrate in bacteria, which is crucial for nitrogen cycling in aquatic and terrestrial ecosystems. This antibody specifically targets the Nitrific-γ protein, enabling researchers to detect and analyze its expression in different cell types and environmental samples.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
pan-Nitrific-Y Antibody
SKU:
CAB20441
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
HeLa treated with Perxynitrate, C6 treated with Perxynitrate, NIH/3T3 treated with Perxynitrate
Observed MW:
10-250kDa
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using pan-Nitrific-Y Rabbit pAb (CAB20441) at 1:1000 dilution. HeLa and C6 cells were treated with Perxynitrate（3mM）for 1 hours. Jurkat cells were treated with Pervanadate (1 mM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from NIH/3T3 cells, using pan-Nitrific-Y Rabbit pAb (CAB20441) at 1:1000 dilution. NIH/3T3 cells were treated with Perxynitrate（3mM）for 3 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
