Based on the information in the provided URL for the Anti-Phospho-DOK2 (Y299) Antibody (CABP0250), this product is designed for research involving DOK2, a signaling protein that plays a crucial role in various cellular processes. The antibody, produced using advanced technology, specifically targets the phosphorylated form of DOK2 at tyrosine 299, allowing for precise detection and analysis in research applications.Phosphorylation of DOK2 at tyrosine 299 is known to regulate its function in signaling pathways related to cell growth, differentiation, and survival. The antibody is validated for use in various experimental techniques, including Western blotting, immunofluorescence, and immunoprecipitation, making it a versatile tool for studies investigating the role of DOK2 phosphorylation in cellular signaling pathways.
Understanding the mechanisms underlying DOK2 phosphorylation is essential for deciphering its involvement in diseases such as cancer, immune disorders, and developmental abnormalities. This antibody provides a valuable resource for researchers interested in unraveling the complex regulatory networks that govern cellular signaling and identifying potential therapeutic targets for a wide range of pathological conditions.
Product Name:
Phospho-DOK2-Y299 Rabbit Polyclonal Antibody
SKU:
CABP0250
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding Y299 of human DOK2
Tested Applications:
WBIF/ICC
Recommended Dilution:
WB,1:500 - 1:2000 IF/ICC,1:100 - 1:200
Synonyms:
p56DOK; p56dok-2; Phospho-DOK2-Y299
Positive Sample:
Jurkat
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol
Calculated MW:
45kDa
Observed MW:
55kDa
The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells.
Purification Method:
Affinity purification
Gene ID:
9046
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells using Phospho-DOK2-Y299 Rabbit pAb (CABP0250).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
