The Phospho-Histone H3-T32 Antibody (CABP0897) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically targets phosphorylated Histone H3 at Threonine 32, a key modification associated with mitosis and chromosome condensation. Developed using advanced technology, this antibody offers high specificity and sensitivity for detecting phosphorylated Histone H3 at T32 in a variety of samples. It has been rigorously validated for use in techniques such as immunofluorescence, immunoprecipitation, and flow cytometry, providing reliable results for researchers investigating cell cycle progression, proliferation, and epigenetic modifications.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Phospho-Histone H3-T32 Antibody
SKU:
CABP0897
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PATG G
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
RRID
AB_2863816
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-Histone H3-T32 Rabbit pAb (CABP0897) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using Phospho-Histone H3-T32 Rabbit pAb (CABP0897) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
