The Anti-PIF4 Antibody (CAB20725) is a valuable tool for researchers investigating the PIF4 protein, a key regulator of plant growth and development. This polyclonal antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting PIF4 in plant samples, particularly in Western blot applications. By targeting the PIF4 protein, this antibody allows for the accurate identification and analysis of PIF4 expression levels in different plant species and tissues.PIF4, also known as phytochrome-interacting factor 4, plays a crucial role in mediating light signaling and regulating various physiological processes in plants, such as seed germination, seedling growth, and flowering time.
Understanding the function and regulation of PIF4 is essential for unraveling the molecular mechanisms that control plant development and environmental responses. Research on PIF4 may lead to advancements in crop improvement, stress tolerance, and overall plant productivity.Overall, the Anti-PIF4 Antibody (CAB20725) serves as a valuable tool for researchers interested in unraveling the complex regulatory networks involving the PIF4 protein and its impact on plant growth and development. Its high specificity and sensitivity make it an excellent choice for studies in plant biology, agriculture, and biotechnology.
Antibody Name:
Anti-PIF4 Antibody (CAB20725)
Antibody SKU:
CAB20725
Antibody Size:
50µL, 100µL
Application:
Western blotting
Reactivity:
Arabidopsis thaliana
Host Species:
Rabbit
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of arabidopsis thaliana PIF4 (NP_565991.2).
Application:
Western blotting
Recommended Dilution:
WB 1:500 - 1:2000
Reactivity:
Arabidopsis thaliana
Positive Samples:
seedling, rosette leaf
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of arabidopsis thaliana PIF4 (NP_565991.2).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isolated as a semidominant mutation defective in red -light responses. Encodes a nuclear localized bHLH protein that interacts with active PhyB protein. Negatively regulates phyB mediated red light responses. Involved in shade avoidance response. Protein abundance is negatively regulated by PhyB.
Western blot analysis of extracts of various cell lines, using PIF4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
