The PLA2G2A Monoclonal Antibody (CAB19252) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot experiments. By binding to the PLA2G2A protein, this antibody allows for precise detection and analysis in various cell types, making it an ideal choice for immunology and cancer research studies.PLA2G2A is a crucial enzyme involved in the production of lipid mediators that regulate inflammation and immune responses.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PLA2G2A Monoclonal Antibody
SKU:
CAB19252
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2416
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Rat small intestine, Rat liver, HT-29, Hep G2, Mouse liver, Mouse spleen
Cellular Localization:
Membrane, Peripheral Membrane Protein.
Calculated MW:
16kDa
Observed MW:
15kDa
The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.
Purification Method
Affinity purification
Gene ID
5320
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PLA2G2A Rabbit mAb (CAB19252) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5 s.
Western blot analysis of various lysates using PLA2G2A Rabbit mAb (CAB19252) at 1:4000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
