The PRMT7 Monoclonal Antibody (CAB20975) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, exhibits high specificity and sensitivity towards PRMT7 in human samples, making it an excellent choice for Western blot applications.PRMT7, a member of the protein arginine methyltransferase family, is known to participate in the methylation of various proteins, thereby affecting their functions and interactions within the cell. Its role in epigenetic regulation and post-translational modifications makes it a key player in cell biology research, particularly in fields like cancer biology and developmental biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PRMT7 Monoclonal Antibody
SKU:
CAB20975
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2967
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SBIDDS, PRMT7
Positive Sample:
HeLa, SW620, MCF7, NIH/3T3, Mouse testis, Mouse brain, Rat testis, Rat brain
Cellular Localization:
Cytoplasm, Nucleus, Cytosol.
Calculated MW:
78kDa
Observed MW:
78kDa
This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients.
Purification Method
Affinity purification
Gene ID
54496
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PRMT7 Rabbit mAb (CAB20975) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of various lysates using PRMT7 Rabbit mAb (CAB20975) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
