Description
RPL7A Monoclonal Antibody (CAB19247)
The RPL7A Monoclonal Antibody (CAB19247) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the RPL7A protein, this antibody enables accurate detection and analysis in various cell types, making it a versatile option for studies in molecular biology and cancer research.RPL7A plays a crucial role in the formation of the ribosome, the cellular machinery responsible for protein synthesis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | RPL7A Monoclonal Antibody |
| SKU: | CAB19247 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Clone Number: | ARC2407 |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | MPKG KKAK GKKV APAP AVVK KQEA KKVV NPLF EKRP KNFG IGQD IQPK RDLT RFVK WPRY IRLQ RQRA ILYK RLKV PPAI NQFT QALD RQTA TQLL KLAH KYRP ETKQ EKKQ RLLA RAEK KAAG KGDV PTKR PPVL RAGV NTVT TLVE NKKA QLVV IAHD VDPI E | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | L7A, eL8, TRUP, SURF3, PLA-X polypeptide, RPL7A |
| Positive Sample: | 293T, PC-12, Hep G2, NIH/3T3, Raji, Mouse lung, Mouse liver, Rat lung |
| Cellular Localization: | Cytoplasm, Cytosol, Cytosolic Ribosome, Focal Adhesion, Nucleolus, Nucleus. |
| Calculated MW: | 30kDa |
| Observed MW: | 30kDa |
Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene to form the chimeric oncogene trk-2h, which encodes an oncoprotein consisting of the N terminus of ribosomal protein L7a fused to the receptor tyrosine kinase domain of trk. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
| Purification Method | Affinity purification |
| Gene ID | 6130 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using RPL7A Rabbit mAb (CAB19247) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s. |
