The SAE1 Antibody (CAB9960) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it an ideal tool for studying the role of SAE1 in various cellular processes.SAE1 is a critical enzyme involved in the SUMOylation pathway, which regulates protein function and cellular signaling. Research has shown that dysregulation of the SUMOylation pathway can contribute to diseases such as cancer, neurodegenerative disorders, and viral infections.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SAE1 Antibody
SKU:
CAB9960
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AOS1, SUA1, UBLE1A, HSPC140, SAE1
Positive Sample:
HeLa, B cells, K-562, HT-29, LO2, NIH/3T3
Cellular Localization:
Nucleus.
Calculated MW:
38kDa
Observed MW:
38kDa
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).
Purification Method
Affinity purification
Gene ID
10055
RRID
AB_2772138
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SAE1 Rabbit pAb (CAB9960) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
