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Anti-SAE1 Antibody (CAB9960)

SKU:
CAB9960
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-SAE1 Antibody
Antibody SKU:CAB9960
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human SAE1 (NP_005491.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, B cells, K-562, HT-29, LO2, NIH/3T3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human SAE1 (NP_005491.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVEK EEAG GGIS EEEA AQYD RQIR LWGL EAQK RLRA SRVL LVGL KGLG AEIA KNLI LAGV KGLT MLDH EQVT PEDP GAQF LIRT GSVG RNRA EASL ERAQ NLNP MVDV KVDT EDIE KKPE SFFT QFDA VCLT CCSR DVIV KVDQ ICHK NSIK FFTG DVFG YHGY TFAN LGEH EFVE EKTK VAKV SQGV EDGP DTKR AKLD SSET TMVK KKVV FCPV KEAL EVDW SSEK AKAA LKRT TSDY FLLQ VLLK FRTD KGRD PSSD TYEE DSEL LLQI RNDV LDSL GISP DLLP EDFV RYCF SEMA PVCA VVGG ILAQ EIVK ALSQ RDPP HNNF FFFD GMKG NGIV ECLG PK
Gene ID:10055
Uniprot:Q9UBE0
Cellular Location:Nucleus
Calculated MW:29kDa/33kDa/38kDa
Observed MW:38kDa
Synonyms:SAE1, AOS1, HSPC140, SUA1, UBLE1A
Background:
UniProt Protein Function:UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family.
UniProt Protein Details:

Protein type:Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function:ATP-dependent protein binding; enzyme activator activity; protein binding; protein C-terminus binding; protein heterodimerization activity; SUMO activating enzyme activity; ubiquitin activating enzyme activity

Biological Process: protein sumoylation; protein ubiquitination; regulation of mitotic cell cycle

NCBI Summary:Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
UniProt Code:Q9UBE0
NCBI GenInfo Identifier:42559897
NCBI Gene ID:10055
NCBI Accession:Q9UBE0.1
UniProt Secondary Accession:Q9UBE0,O95717, Q9P020, B2RDP5, B3KMQ2, F5GXX7, G3XAK6
UniProt Related Accession:Q9UBE0
Molecular Weight:33,063 Da
NCBI Full Name:SUMO-activating enzyme subunit 1
NCBI Synonym Full Names:SUMO1 activating enzyme subunit 1
NCBI Official Symbol:SAE1  
NCBI Official Synonym Symbols:AOS1; SUA1; UBLE1A; HSPC140  
NCBI Protein Information:SUMO-activating enzyme subunit 1
UniProt Protein Name:SUMO-activating enzyme subunit 1
UniProt Synonym Protein Names:Ubiquitin-like 1-activating enzyme E1A
Protein Family:SUMO-activating enzyme
UniProt Gene Name:SAE1  
UniProt Entry Name:SAE1_HUMAN
Anti-SAE1 Polyclonal Antibody (CAB9960)Western blot analysis of extracts of various cell lines, using SAE1 antibody (CAB9960) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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