Description
SARS-CoV-2 3CLpro Antibody (CAB20198)
The SARS-CoV-2 3CLpro Antibody (CAB20198) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically targets the 3CLpro protein of the virus and is highly reactive with human samples. It has been validated for use in various applications, including Western blotting, enabling researchers to detect and analyze the 3CLpro protein in different cell types.The 3CLpro protein, also known as the main protease, plays a vital role in viral replication and is a potential target for antiviral drug development.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against SARS-CoV-2 samples.
| Product Name: | SARS-CoV-2 3CLpro Antibody |
| SKU: | CAB20198 |
| Size: | 20μL, 100μL |
| Reactivity: | SARS-CoV-2 |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | MESL VPGF NEKT HVQL SLPV LQVR DVLV RGFG DSVE EVLS EARQ HLKD GTCG LVEV EKGV LPQL EQPY VFIK RSDA RTAP HGHV MVEL VAEL EGIQ YGRS GETL GVLV PHVG EIPV AYRK VLLR KNGN KGAG GHSY GADL KSFD LGDE LGTD PYED FQEN WNTK HSSG VTRE LMRE LNGG AYTR YVDN NFCG PDGY PLEC IKDL LARA GKAS CTLS EQLD FIDT KRGV YCCR EHEH EIAW YTER SEKS YELQ TPFE IKLA KKFD TFNG ECPN FVFP LNSI IKTI QPRV EKKK LDGF MGRI RSVY PV | ||||||
| Tested Applications: | WB IF/ICC ELISA | ||||||
| Recommended Dilution: |
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| Positive Sample: | 293T |
| Calculated MW: | 141kDa |
| Observed MW: | 30-35kDa |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.
| Purification Method | Affinity purification |
| Gene ID | 43740578 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from 293T cells, using SARS-CoV-2 3CLpro Rabbit pAb (CAB20198) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s. |
 | Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 3CLpro fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 3CLpro Rabbit pAb (CAB20198) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
