Description
SARS-CoV-2 NSP1 Antibody (CAB20200)
The SARS-CoV-2 NSP1 Antibody (CAB20200) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, raised in rabbits, is highly reactive with samples from individuals infected with SARS-CoV-2 and is validated for use in various applications, including Western blotting.The NSP1 protein of SARS-CoV-2 is a non-structural protein involved in viral replication and immune evasion. By targeting this specific protein, researchers can gain insights into how the virus manipulates host cells and evades the immune response.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against SARS-CoV-2 samples.
| Product Name: | SARS-CoV-2 NSP1 Antibody |
| SKU: | CAB20200 |
| Size: | 20μL, 100μL |
| Reactivity: | SARS-CoV-2 |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | MESL VPGF NEKT HVQL SLPV LQVR DVLV RGFG DSVE EVLS EARQ HLKD GTCG LVEV EKGV LPQL EQPY VFIK RSDA RTAP HGHV MVEL VAEL EGIQ YGRS GETL GVLV PHVG EIPV AYRK VLLR KNGN KGAG GHSY GADL KSFD LGDE LGTD PYED FQEN WNTK HSSG VTRE LMRE LNGG | ||||||
| Tested Applications: | WB IP ELISA | ||||||
| Recommended Dilution: |
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| Positive Sample: | 293T |
| Calculated MW: | 141kDa |
| Observed MW: | 20kDa |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.
| Purification Method | Affinity purification |
| Gene ID | 43740578 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of extracts of normal 293T cells 293T transfected with NSP1 Protein, using SARS-CoV-2 NSP1 Rabbit pAb (CAB20200) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s. |
 | Immunoprecipitation analysis of 600 μg extracts of 293T cells using 3 μg SARS-CoV-2 NSP1 antibody (CAB20200). Western blot was performed from the immunoprecipitate using SARS-CoV-2 NSP1 antibody (CAB20200) at a dilution of 1:1000. |
