Description
SARS-CoV-2 NSP13 Antibody (CAB20311)
The SARS-CoV-2 NSP13 Antibody (CAB20311) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced using advanced technology, is highly specific and sensitive for detecting the NSP13 protein associated with the SARS-CoV-2 virus. NSP13 is a helicase enzyme that plays a key role in viral RNA replication and transcription, making it an attractive target for antiviral drug development. By using this antibody in various research applications such as Western blotting and immunofluorescence, scientists can gain valuable insights into the biology of the virus and potentially identify new therapeutic strategies to combat COVID-19.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against SARS-CoV-2 samples.
| Product Name: | SARS-CoV-2 NSP13 Antibody |
| SKU: | CAB20311 |
| Size: | 20μL, 100μL |
| Reactivity: | SARS-CoV-2 |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | AVGA CVLC NSQT SLRC GACI RRPF LCCK CCYD HVIS TSHK LVLS VNPY VCNA PGCD VTDV TQLY LGGM SYYC KSHK PPIS FPLC ANGQ VFGL YKNT CVGS DNVT DFNA IATC DWTN AGDY ILAN TCTE RLKL FAAE TLKA TEET FKLS YGIA TVRE VLSD RELH LSWE VGKP RPPL NRNY VFTG YRVT KNSK VQIG EYTF EKGD YGDA VVYR GTTT YKLN VGDY FVLT SHTV MPLS APTL VPQE HYVR ITGL YPTL NISD EFSS NVAN YQKV GMQK YSTL QGPP GTGK SHFA IGLA LYYP SARI VYTA CSHA AVDA LCEK ALKY LPID KCSR IIPA RARV ECFD KFKV NSTL EQYV FCTV NALP ETTA DIVV FDEI SMAT NYDL SVVN ARLR AKHY VYIG DPAQ LPAP RTLL TKGT LEPE YFNS VCRL MKTI GPDM FLGT CRRC PAEI VDTV SALV YDNK LKAH KDKS AQCF KMFY KGVI THDV SSAI NRPQ IGVV REFL TRNP AWRK AVFI SPYN SQNA VASK ILGL PTQT VDSS QGSE YDYV IFTQ TTET AHSC NVNR FNVA ITRA KVGI LCIM SDRD LYDK LQFT SLEI PRRN VATL Q | ||||||
| Tested Applications: | WB IF/ICC ELISA | ||||||
| Recommended Dilution: |
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| Positive Sample: | 293T |
| Calculated MW: | 794kDa |
| Observed MW: | 75kDa |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.
| Purification Method | Affinity purification |
| Gene ID | 43740578 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of normal 293T cells and 293T transfected with NSP13 Protein, using SARS-CoV-2 NSP13 Rabbit pAb (CAB20311) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s. |
 | Immunofluorescence analysis of 293T-SARS-CoV-2 NSP13(His-tag) and 293T cells using SARS-CoV-2 NSP13 Rabbit pAb (CAB20311) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
