Description
SARS-CoV Spike RBD Antibody (CAB20606)
The SARS-CoV Spike RBD Antibody (CAB20606) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with samples from SARS-CoV-infected individuals, making it an essential asset in studying viral pathogenesis and developing diagnostics and therapeutics.The Spike RBD is a key component of the SARS-CoV virus that plays a vital role in viral entry into host cells. Understanding the interaction between the Spike RBD and host cell receptors is essential for unraveling the mechanisms of viral infection and developing effective countermeasures.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against SARS-CoV samples.
| Product Name: | SARS-CoV Spike RBD Antibody |
| SKU: | CAB20606 |
| Size: | 20μL, 100μL |
| Reactivity: | SARS-CoV |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | ADYS VLYN STFF STFK CYGV SATK LNDL CFSN VYAD SFVV KGDD VRQI APGQ TGVI ADYN YKLP DDFM GCVL AWNT RNID ATST GNYN YKYR YLRH GKLR P | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | E2, SARS-CoV Spike RBD |
| Positive Sample: | 293T |
| Calculated MW: | 139kDa |
| Observed MW: | 200kDa |
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor.
| Purification Method | Affinity purification |
| Gene ID | 1489668 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using SARS-CoV Spike RBD Rabbit pAb (CAB20606) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s. |
