The SARS-CoV-2 Spike S1 Monoclonal Antibody (CAB20022) is a high-quality antibody developed for reliable detection and analysis of target proteins. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests. RRID AB_2862925 Gene ID 43740568 Swiss Prot Synonym spike glycoprotein
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against SARS-CoV-2 samples.
Product Name:
SARS-CoV-2 Spike S1 Monoclonal Antibody
SKU:
CAB20022
Size:
100μL, 20μL
Reactivity:
SARS-CoV-2
Clone Number:
ARC2373
Conjugate:
-
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:2000 - 1:10000
IF
/
ICC
1:200 - 1:800
ELISA
1:1000 - 1:5000
Synonyms:
spike glycoprotein
Positive Sample:
293T cells transfected with SARS-CoV-2 Spike S1 protein
Cellular Localization:
-
Calculated MW:
-
Observed MW:
110kDa
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests. RRID AB_2862925 Gene ID 43740568 Swiss Prot Synonym spike glycoprotein
Purification Method:
Affinity purification
Gene ID:
43740568
RRID:
AB_2862925
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SARS-CoV-2 Spike S1 using SARS-CoV-2 Spike S1 mAb (CAB20022) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 5μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180 s.
