The SPOP Monoclonal Antibody (CAB19578) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for use in Western blot applications. By binding to the SPOP protein, researchers can effectively analyze and detect its expression in various cell types, providing valuable insights into its role in cancer progression and therapeutic resistance.SPOP, also known as Speckle-type POZ protein, is a key regulator of protein degradation and stability, influencing various cellular processes such as cell growth, proliferation, and differentiation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SPOP Monoclonal Antibody
SKU:
CAB19578
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2181
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.
Purification Method
Affinity purification
Gene ID
8405
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from PC-3 cells, using SPOP Rabbit mAb (CAB19578) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
