The Steroidogenic factor-1 (SF-1/NR5A1) Monoclonal Antibody (CAB19277) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits a high degree of reactivity with human samples and has been rigorously validated for use in Western blotting applications. By specifically targeting the SF-1/NR5A1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an indispensable resource for studies in endocrinology, reproductive biology, and developmental biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect.
Purification Method
Affinity purification
Gene ID
2516
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Steroidogenic factor-1 (SF-1/NR5A1) Rabbit mAb (CAB19277) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
