The TIGIT Antibody (CAB20350) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, is highly specific for human samples and is validated for use in Western blot applications.TIGIT, also known as T cell immunoreceptor with Ig and ITIM domains, plays a crucial role in immune responses by inhibiting T cell activation and promoting immune tolerance. Understanding the function of TIGIT is essential for research in immunology and cancer, as it is implicated in the regulation of immune checkpoints and the suppression of anti-tumor immune responses.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
TIGIT Antibody
SKU:
CAB20350
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VSIG9, VSTM3, WUCAM, TIGIT
Positive Sample:
Human plasma, Mouse lung
Cellular Localization:
Cell Surface, Plasma Membrane.
Calculated MW:
26kDa
Observed MW:
30kDa
This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.
Purification Method
Affinity purification
Gene ID
201633
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TIGIT Rabbit pAb (CAB20350) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
