The TPMT Monoclonal Antibody (CAB20922) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blotting applications.TPMT plays a crucial role in determining the efficacy and toxicity of thiopurine medications, making it a focal point of study in pharmacogenetics and personalized medicine. Understanding the expression and activity of TPMT in different cell types and tissues is essential for optimizing drug dosages and minimizing adverse reactions in patients undergoing thiopurine therapy.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TPMT Monoclonal Antibody
SKU:
CAB20922
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2897
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TPMTD, TPMT
Positive Sample:
HeLa, 293T, Hep G2, K-562, Mouse liver, Mouse brain, Mouse kidney, Rat liver, Rat kidney
Cellular Localization:
Cytoplasm.
Calculated MW:
28kDa
Observed MW:
28kDa
This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X.
Purification Method
Affinity purification
Gene ID
7172
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TPMT Rabbit mAb (CAB20922) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
