The USP14 Monoclonal Antibody (CAB19589) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it an ideal choice for Western blot applications. By binding to USP14, researchers can easily detect and study its expression and activity in various cell types.USP14, a deubiquitinating enzyme, plays a crucial role in the regulation of protein turnover and quality control in cells. Dysregulation of USP14 has been linked to various diseases, including neurodegenerative disorders and cancer.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
USP14 Monoclonal Antibody
SKU:
CAB19589
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2185
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method
Affinity purification
Gene ID
9097
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using USP14 Rabbit mAb (CAB19589) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg USP14 antibody (CAB19589). Western blot was performed from the immunoprecipitate using USP14 antibody (CAB19589) at a dilution of 1:2000.
