The USP39 Monoclonal Antibody (CAB1393) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable thiol-dependent deubiquitinase and zinc ion binding activity. Involved in spliceosomal complex assembly. Located in nucleoplasm. Part of U4/U6 x U5 tri-snRNP complex.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
USP39 Monoclonal Antibody
SKU:
CAB1393
Size:
100μL, 20μL
Reactivity:
Human, Rat
Clone Number:
ARC2565
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
65K, SAD1, CGI-21, HSPC332, SNRNP65, USP39
Positive Sample:
HCT116
Cellular Localization:
Nucleus.
Calculated MW:
65kDa
Observed MW:
65kDa
Predicted to enable thiol-dependent deubiquitinase and zinc ion binding activity. Involved in spliceosomal complex assembly. Located in nucleoplasm. Part of U4/U6 x U5 tri-snRNP complex.
Purification Method
Affinity purification
Gene ID
10713
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HCT116 cells, using USP39 Rabbit mAb (CAB1393) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using USP39 Rabbit mAb (CAB1393) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
