The VPS18 Monoclonal Antibody (CAB17563) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, is highly specific for VPS18 and is suitable for use in various experimental techniques, including Western blotting and immunofluorescence.VPS18 plays a crucial role in endosomal sorting and autophagy, making it an essential player in cellular homeostasis and protein degradation pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
VPS18 Monoclonal Antibody
SKU:
CAB17563
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2156
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PEP3, VPS18
Positive Sample:
A549
Cellular Localization:
Autophagosome, Clathrin-Coated Vesicle, Corvet Complex, Early Endosome, Endosome, Glutamatergic Synapse, Late Endosome, Lysosome, Presynapse.
Calculated MW:
110kDa
Observed MW:
110kDa
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.
Purification Method
Affinity purification
Gene ID
57617
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A549 cells using VPS18 Rabbit mAb (CAB17563) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20 s.
