The APOA4 Monoclonal Antibody (CAB23893) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in mouse, is highly specific for detecting ApoA4 in human samples and is suitable for use in various laboratory techniques, including Western blot and immunohistochemistry.ApoA4 is known to play a role in the transport of lipids, including cholesterol, in the blood. It has been linked to the regulation of lipid levels and may have protective effects against atherosclerosis and cardiovascular disease.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
APOA4 Monoclonal Antibody
SKU:
CAB23893
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC62234
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
APOA4
Positive Sample:
Hep G2
Cellular Localization:
Secreted.
Calculated MW:
45kDa
Observed MW:
45kDa
Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.
Purification Method
Affinity purification
Gene ID
337
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using APOA4 Rabbit mAb (CAB23893) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): THP-1 Exposure time: 45 s.
