The APTX Antibody (CAB5364) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By targeting the aprataxin protein, this antibody enables precise detection and analysis in a wide range of cell types, making it an invaluable asset for studies in DNA repair mechanisms and related diseases.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
APTX Antibody
SKU:
CAB5364
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AOA, AOA1, AXA1, EAOH, EOAHA, FHA-HIT, APTX
Positive Sample:
22Rv1, A-549, K-562, BT-474, U-87MG, HT-29, Raji
Cellular Localization:
Cytoplasm, Nucleus, Nucleolus, Nucleoplasm.
Calculated MW:
41kDa
Observed MW:
40kDa
This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.
Purification Method
Affinity purification
Gene ID
54840
RRID
AB_2766174
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using APTX Rabbit pAb (CAB5364) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A549 cells using APTX Rabbit pAb (CAB5364).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
