The Aquaporin-1 (AQP1) Antibody (CAB15030) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a small integral membrane protein with six bilayer spanning domains that functions as a water channel protein. This protein permits passive transport of water along an osmotic gradient. This gene is a possible candidate for disorders involving imbalance in ocular fluid movement.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Aquaporin-1 (AQP1) Antibody
SKU:
CAB15030
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISAIF-P
Recommended Dilution:
WB
1:100 - 1:500
IF-P
1:50 - 1:200
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CO, CHIP28, AQP-CHIP, Aquaporin-1 (AQP1)
Positive Sample:
Mouse liver, Mouse heart, Rat lung
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
29kDa
Observed MW:
25kDa
This gene encodes a small integral membrane protein with six bilayer spanning domains that functions as a water channel protein. This protein permits passive transport of water along an osmotic gradient. This gene is a possible candidate for disorders involving imbalance in ocular fluid movement.
Purification Method
Affinity purification
Gene ID
358
RRID
AB_2761910
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Aquaporin-1 (AQP1) Rabbit pAb (CAB15030) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human kidney using Aquaporin-1 (AQP1) Rabbit pAb (CAB15030) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using Aquaporin-1 (AQP1) Rabbit pAb (CAB15030) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded rat kidney using Aquaporin-1 (AQP1) Rabbit pAb (CAB15030) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
