The AR Polyclonal Antibody (CAB16200) is a valuable tool for researchers studying the AR protein, a key regulator of androgen receptor signaling pathways. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the AR protein, it enables accurate detection and analysis in various cell types, making it ideal for studies in cancer biology, endocrinology, and developmental biology.The androgen receptor (AR) is a ligand-activated transcription factor that plays a crucial role in mediating the effects of androgens in both normal development and disease.
Dysregulation of the AR signaling pathway is associated with the progression of prostate cancer, making it a promising target for therapeutic intervention. Research into the molecular mechanisms underlying AR function is essential for the development of novel therapies and diagnostic tools for prostate cancer and other androgen-related disorders. The AR Polyclonal Antibody (CAB16200) provides researchers with a reliable tool to further investigate the intricate role of the AR protein in these processes.
Product Name:
Androgen Receptor Rabbit Polyclonal Antibody
SKU:
CAB16200
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Androgen Receptor (NP_000035.2).
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
367
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Androgen Receptor antibody (CAB16200) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 40s.
