The ARAF Antibody (CAB16346) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables protein serine/threonine kinase activity. Involved in negative regulation of apoptotic process; regulation of TOR signaling; and regulation of cellular protein metabolic process. Predicted to be active in cytosol and mitochondrion. Biomarker of high grade glioma. RRID AB_2768411 Gene ID 369 Swiss Prot Synonym PKS2; A-RAF; ARAF1; RAFA1; ARAF
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ARAF Antibody
SKU:
CAB16346
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PKS2, A-RAF, ARAF1, RAFA1, ARAF
Positive Sample:
-
Cellular Localization:
Cytosol, Mitochondrion.
Calculated MW:
68kDa
Observed MW:
Refer to figures
Enables protein serine/threonine kinase activity. Involved in negative regulation of apoptotic process; regulation of TOR signaling; and regulation of cellular protein metabolic process. Predicted to be active in cytosol and mitochondrion. Biomarker of high grade glioma. RRID AB_2768411 Gene ID 369 Swiss Prot Synonym PKS2; A-RAF; ARAF1; RAFA1; ARAF
Purification Method:
Affinity purification
Gene ID:
369
RRID:
AB_2768411
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ARAF Rabbit pAb (CAB16346) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using ARAF Rabbit pAb (CAB16346) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
