The ARFGAP1 Antibody (CAB7118) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the ARFGAP1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for research in cell biology and molecular biology.ARFGAP1 is a key player in maintaining cellular function by controlling the movement of proteins and lipids between different compartments within the cell. Dysregulation of ARFGAP1 has been linked to various diseases, including cancer and neurological disorders, making it an important target for drug development and therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ARFGAP1 Antibody
SKU:
CAB7118
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ARF1GAP, HRIHFB2281, ARFGAP1
Positive Sample:
BT-474, HeLa, HepG2, MCF7, Human placenta, Mouse liver, Mouse ovary, Mouse brain, Rat lung
Cellular Localization:
Cytoplasm, Golgi Apparatus.
Calculated MW:
45kDa
Observed MW:
45kDa
The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
55738
RRID
AB_2767673
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ARFGAP1 Rabbit pAb (CAB7118) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
