The ARHGAP1 Antibody (CAB3739) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By targeting the ARHGAP1 protein, this antibody allows for the detection and analysis of ARHGAP1 in a variety of cell types, making it ideal for studies in cell biology and cancer research.ARHGAP1, also known as Rho GTPase-activating protein 1, plays a crucial role in controlling the activity of Rho GTPases, which are key regulators of cell shape, movement, and division.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ARHGAP1 Antibody
SKU:
CAB3739
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RHOGAP, RHOGAP1, CDC42GAP, p50rhoGAP, ARHGAP1
Positive Sample:
BxPC-3, HeLa, HepG2, U-87MG, PC-12, Mouse lung
Cellular Localization:
Cytoplasm.
Calculated MW:
50kDa
Observed MW:
50kDa
This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members.
Purification Method
Affinity purification
Gene ID
392
RRID
AB_2765241
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ARHGAP1 Rabbit pAb (CAB3739) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg ARHGAP1 antibody (CAB3739). Western blot was performed from the immunoprecipitate using ARHGAP1 antibody (CAB3739) at a dilution of 1:1000.
