The ARHGAP25 Antibody (CAB13664) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to ARHGAP25, this antibody enables precise detection and analysis of the protein in various cell types, making it an essential component for studies in cell biology and cancer research.ARHGAP25, a Rho GTPase activating protein, is known for its involvement in controlling the activity of Rho GTPases, which are crucial regulators of cell morphology and motility.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ARHGAP25 Antibody
SKU:
CAB13664
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KAIA0053, HEL-S-308, ARHGAP25
Positive Sample:
Jurkat, A-549, Mouse lung, Rat spleen
Cellular Localization:
Phagocytic Cup.
Calculated MW:
73kDa
Observed MW:
73kDa
ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).
Purification Method
Affinity purification
Gene ID
9938
RRID
AB_2760525
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ARHGAP25 Rabbit pAb (CAB13664) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
